ET 33-mi 65A: EGFP expression in fins, sclerotome, skin, heart, liver, swimbladder and gills.

ET 33-mi 65B: EGFP expression in the forebrain (epiphysis), fins, sclerotome and skin.

Insertion 1

Genomic location: Chromosome 20

The insertion is in the intron of snap25a (synaptosome-associated protein 25a)

DNA sequence

Insertion 2

Genomic location: Chromosome 20

The insertion is 21.2 kb upstream of stmn4 (stathmin-like 4)

DNA sequences flanking Tol2 insertion sites

5:TTCACTACAGTATAATCAAATTTAAACTAGATTGCATTCCAGCAGAAGTA

TTTTCATCTTAAAATGAACAGTTGACATGATTATAGTTGGATTTCTATTTCT

ACATATCAAACGCATGCTTTGCACAGGGAGTGTGAGCAGTAAGTGGTTATGA

GACATGTAAATCGAGTGCTAGAATCAGTCCACTGGTGTTATTAATAACTGCT

CATGAAATCAATCAAGCCTGTTCTATGCTCCTCCACAA

5:TGAATGAATGAATAAATGAATTTATGATTGAATGAATGATCAAAGTCATT

TTGTCATTTGGGGACAAATGTAAGCGTTACACAAGAAAATTAGAATAATAAG

GTATTTGTTAGTTTTTAAATTAAAAAAAACCCGGACATGCCTCTGATTGGAA

TATGATAAATGAATGTTACGGCTGTGTTGTTAATTCTCTGATTGCATCTGTC

TTTTCTCACCAAACCCGTGTTCACCAGCTCCCCCAAGTTCAACCCTCTGAAA

TGGAGCTGTGAAAATGTCCATCTGTGTGTGTTTTCCCTTGGCAAACGGAAAG

CATTTCTTAGTGAAGGTCTTGCCCTGCTTCTCTGGAGTAATTGGTTATTTAG

TCAAGATGAATTATAAGTGTCTCTGTTTTCCAAAGAATGTCTGTGTTATCTT

ATGTTGTTGTGGGATGAGCCAGCAGTTTCGCAAAAACAGGGATGCATGTTGT

TGCTAAATGTAAAAGAATTTTGACAGACTTCTTTTGTAAATACTGTAGAATG

ACCGGATAGGATGCTCCATTAAGCCAGTCTGTCATAAAAATATAATTGAAAT

ATCCCCTTTTTATGTTGATAACAGAGCTAATAGAGGTTTAGGTTTGTAAAAT

GTTCAAAAACTGTACAACCAAGACACTGTATCATCCATGAATTCAATCCGGA

CAATGTGGGTGTGC